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. 2017 Mar 27;7:434. doi: 10.1038/s41598-017-00369-4

Figure 2.

Figure 2

Surface rendered confocal laser scanning microscopy (LSM) images of (A) the triple cell co-culture model cultured on a micro-porous membrane insert, and (B) a micro-porous membrane insert that originally had the co-culture system cultured upon it and subsequently having been treated with Trypsin-EDTA for ≥10 minutes at 37 °C, 5% CO2. Image (B) indicates that the Trypsin-EDTA was successful in detaching the majority of the co-culture from the micro-porous membrane insert in order to form a multi-cell suspension. Image A shows the morphology of the co-culture system when grown under normal culture conditions. Both images show staining for the F-actin cytoskeleton (Phalloidin-Rhodamine) and nuclear (DAPI) regions respectively. Scale bars represent 30 µm. In both (A and B), images show the XY plane in a 3D rendered format (as computed using the software IMARIS®, Switzerland), as well as planar views of XZ and YZ shown in 2D.