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. 2017 Mar 27;7:434. doi: 10.1038/s41598-017-00369-4

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Percentage frequency (%Frequency) of specific surface marker expression upon human blood monocyte derived macrophages (MDM) and dendritic cells (MDDC), as well as A549 epithelial cells when present in the multi-cell model suspension culture. In (A) CD14, Pan-Cytokeratin (A549 epithelial cells) and CD1c and (B) CD11a (MDM), E-Cadherin (A549 epithelial cells), CD80 (MDDC) show the average %Frequency for the antibody master-mix used (please refer to SI Table 1 for all antibody information). Data was obtained after implementing the specific gating strategy for the co-culture suspension (SI Fig. 1B) (C). Samples were analysed via three-colour flow cytometry (FACS). All data was analysed using FlowJo (Version 10, TreeStar, USA). In (A and B) all data expressed is the mean ± standard error of the mean (SEM) of the %Frequency. Experimentation was repeated on three separate occasions in triplicate (n = 3).