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. 2017 Mar 22;7:230. doi: 10.1038/s41598-017-00377-4

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© The Author(s) 2017

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Wnt3a overexpression exacerbates liver fibrosis caused by S. japonicum infection. Mice received either Lv-EGFP or Lv-Wnt3a-EGFP after S. japonicum infection for 4 or 6 weeks. (A) Masson’s trichrome staining of the liver sections was used to measure collagen fibre deposition, and the ratio of the collagen-stained area to the total area was also quantified. (B,C) Hepatic expression of fibrotic markers (Col1a1, Desmin, and α-SMA/Acta2) was assessed by immunohistochemical staining and western blotting. Sj, S. japonicum. Scale bar, 100 μm. Data represent the mean ± SEM; n = 5 (*P < 0.05).