Table 12.

Confirmation of selected genes by qRT-PCR analysis.

Gene	Function	Fold change (45°C, 45 min)
		RT-PCR*	Microarray
bioB	Biotin synthetase, biotin synthesis	172.7 (± 34.5)	23.4
dgoK	2-dehydro-3-deoxygalactonokinase hexonate metabolism	240.2 (± 44.3)	31.1
fes	Ferric enterobactin esterase, iron uptake	285.5 (± 87.5)	11.9
ftnA	Ferritin, iron storage	0.76 (± 0.04)	0.19
iroB	Salmochelin synthesis, iron uptake	14.9 (± 1.4)	4.1
spy	Spheroplast formation	64.5 (± 32.7)	22.2

Highlighted fold changes correspond to up-regulated (dark gray) and down-regulated (light gray) genes.

^*Calculation of fold change by qRT-PCR was determined from the number of RNA copies after 45 min of incubation divided by the number of RNA copies at T = 0). A total of two RNA extractions were performed using distinct S. Enteritidis cultures grown on different batches of EWMM. Each of the two RNA extracts thus obtained was subject to qRT-PCR, in triplicate, for each gene. Data were normalized using values for three internal control genes (asmA, emrA, orf32). Standard deviations are calculated from two sets of triplicate data are in parentheses.