Table 7.
Metabolism and transport of amino acids.
| Gene | Function | Fold Change | ||
|---|---|---|---|---|
| 7 min | 25 min | 45 min | ||
| BRANCHED CHAIN AMINO ACIDS | ||||
| ilvC | Ketol-acid reductoisomerase | 0.14 | 0.09 | 0.06 |
| ilvD | Dihydroxy-acid dehydratase | n.s. | 0.70 | 0.46 |
| ilvE | Branched-chain amino acid aminotransferase | 0.42 | 0.45 | 0.36 |
| ilvG | Acetolactate synthase 2 catalytic subunit | 0.50 | 0.49 | 0.41 |
| ilvM | Acetolactate synthase 2 regulatory subunit | 0.50 | 0.49 | 0.37 |
| leuA | 2-isopropylmalate synthase | n.s. | 0.38 | 0.16 |
| leuB | 3-isopropylmalate dehydrogenase | n.s. | 0.38 | 0.17 |
| leuC | Isopropylmalate isomerase large subunit | n.s. | 0.45 | 0.18 |
| leuD | Isopropylmalate isomerase small subunit | n.s. | 0.61 | 0.33 |
| livF | Leucine/isoleucine/valine transporter ATP-binding subunit | n.s. | 0.67 | 0.48 |
| livG | Leucine/isoleucine/valine transporter ATP-binding subunit | n.s. | 0.46 | 0.27 |
| livK | Leucine-specific binding protein | n.s. | 0.39 | 0.22 |
| livM | Leucine/isoleucine/valine transporter permease subunit | n.s. | 0.51 | 0.29 |
| yeaS | Leucine export protein LeuE | 0.54 | 0.45 | 0.32 |
| AROMATIC AMINO ACIDS | ||||
| aroD | 3-dehydroquinate dehydratase | n.s. | n.s. | 0.48 |
| aroP | Aromatic amino acid transporter | n.s. | 0.55 | 0.35 |
| aroG | Phospho-2-dehydro-3-deoxyheptonate aldolase | n.s. | 0.35 | 0.22 |
| mtr | Tryptophan permease | n.s. | 0.37 | 0.26 |
| pheA | Bifunctional chorismate mutase/prephenate dehydratase | 0.27 | 0.35 | 0.36 |
| trpA | Tryptophan synthase subunit alpha | n.s. | 0.61 | 0.34 |
| trpB | Tryptophan synthase subunit beta | n.s. | 0.53 | 0.29 |
| trpC | Bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase | 0.43 | 0.34 | 0.19 |
| trpD | Bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase | n.s. | 0.34 | 0.21 |
| trpE | Anthranilate synthase component I | n.s. | 0.34 | 0.15 |
| THREONINE | ||||
| thrA | Bifunctional aspartokinase I/homeserine dehydrogenase I | 0.29 | 0.28 | 0.15 |
| thrB | Homoserine kinase | 0.33 | 0.38 | 0.22 |
| thrC | Threonine synthase | n.s. | n.s. | 0.38 |
| thrS | Threonyl-tRNA synthetase | 0.16 | 0.19 | 0.20 |
| ARGININE | ||||
| adi | Arginine decarboxylase | 0.54 | 0.30 | 0.24 |
| artM | Arginine transporter permease subunit ArtM | 0.51 | 0.42 | 0.42 |
| yjdE | Arginine:agmatin antiporter | 0.49 | 0.30 | 0.23 |
| CYSTEINE | ||||
| cysA | Sulfate/thiosulfate transporter subunit | 0.36 | 0.34 | 0.22 |
| cysB | Transcriptional regulator CysB | n.s. | 0.38 | 0.21 |
| cysC | Adenylylsulfate kinase | n.s. | 0.61 | 0.3 |
| cysD | Sulfate adenylyltransferase subunit 2 | n.s. | n.s. | 0.41 |
| cysH | Phosphoadenosine phosphosulfate reductase | n.s. | 0.63 | 0.46 |
| cysI | Sulfite reductase subunit beta | n.s. | 0.76 | 0.43 |
| cysJ | Sulfite reductase (NADPH) flavoprotein beta subunit | n.s. | n.s. | 0.42 |
| cysM | Cysteine synthase B | 0.44 | 0.49 | 0.49 |
| cysN | Sulfate adenylyltransferase subunit 1 | n.s. | n.s. | 0.43 |
| cysP | Thiosulfate transporter subunit | n.s. | 0.63 | 0.38 |
| cysU | Sulfate/thiosulfate transporter subunit | n.s. | 0.45 | 0.26 |
| cysW | Sulfate/thiosulfate transporter permease subunit | 0.31 | 0.37 | 0.24 |
| yedO | D-cysteine desulfhydrase | 0.42 | 0.42 | 0.40 |
| ASPARAGINE, GLUTAMATE | ||||
| asnA | Asparagine synthetase AsnA | n.s. | n.s. | 0.25 |
| asnB | Asparagine synthetase B | 0.36 | 0.37 | 0.36 |
| gltB | Glutamate synthase subunit alpha | 0.37 | 0.49 | 0.30 |
| gltD | Glutamate synthase subunit beta | n.s. | 0.68 | 0.41 |
| gltJ | Glutamate/aspartate transport system permease protein GltJ | 0.52 | n.s. | 0.45 |
| gltK | Glutamate/aspartate transport system permease protein GltK | 0.48 | 0.76 | 0.43 |
| gltP | Glutamate/aspartate:proton symporter | 0.55 | 0.54 | 0.40 |
| LYSINE | ||||
| cadB | Lysine/cadaverine antiporter | 0.47 | 0.49 | 0.37 |
| lysA | Diaminopimelate decarboxylase | 0.23 | 0.11 | 0.10 |
| lysC | Aspartate kinase III | 2.77 | 4.14 | 5.99 |
| OTHER AMINO-ACID OR PEPTIDE TRANSPORTERS | ||||
| dppA | Periplasmic dipeptide transport protein precursor | n.s. | 0.35 | 0.19 |
| dppB | Dipeptide transporter permease DppB | 0.37 | 0.40 | 0.24 |
| dppC | Dipeptide transporter | n.s. | n.s. | 0.33 |
| dppD | Dipeptide transporter ATP-binding subunit | 0.40 | 0.51 | 0.30 |
| dppF | Dipeptide transporter ATP-binding subunit | 0.44 | 0.55 | 0.39 |
| oppA | Periplasmic oligopeptide-binding protein precursor (OppA) | n.s. | 0.64 | 0.36 |
| oppB | Oligopeptide transporter permease | n.s. | 0.54 | 0.27 |
| oppC | Oligopeptide transport system permease protein (OppC) | 0.47 | 0.49 | 0.34 |
| oppD | Oligopeptide transporter ATP-binding component | 0.57 | 0.74 | 0.46 |
| yecC | Putative amino-acid ABC transporter ATP-binding protein | 0.41 | 0.41 | 0.40 |
| yecS | Putative ABC transporter membrane protein | 0.38 | 0.40 | 0.41 |
Highlighted fold changes correspond to up-regulated (dark gray) and down-regulated (light gray) genes. (n.s.), fold changes with p > 0.05.