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. 2017 Feb 2;7:24. doi: 10.1038/s41598-017-00043-9

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effects of different normalization strategies on observed Fstl1 expression. Fstl1 expression in whole heart during development (embryonic day 10.5 and 14.5, postnatal day 7 and adult mouse) was determined with qPCR using Ppia and Rpl32 (A) or Gapdh as reference genes for normalization (B). Fstl1 expression in the normal left ventricle and at different days after induced myocardial infarction (MI) using Eef1e1 and Rpl4 (C) or Gapdh (D) as reference genes for normalization. The normalized Follistatin-like 1 expression differs significantly between groups (one-way ANOVA). The insets show the result of the subsequent multiple comparison between groups. Grey boxes indicate subsets of groups with absence of evidence for differential expression (p-value < 0.05) according the Student-Newman-Keuls test.