Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 3;7:45. doi: 10.1038/s41598-017-00079-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

ZEB1 promotes UM cell dedifferentiation by repressing the pigment synthesis pathway. A significant underexpression of genes involving in melanocyte differentiation such as (A) BAP1, (B) MITF, (C) TYR, and (D) TYRP1 was detected in UM and MetUM as compared to NUM. Higher expression of these differentiation genes was also detected in the spindle non-aggressive OCM1 as compared to the epithelioid aggressive C918. (E) ZEB1 was only detected in the epithelioid cells which manifested non- or less pigment in a representative patient UM section. An H&E stained section of the same area is included to show the mixed phenotype. Knockdown of ZEB1 significantly amplified transcription of all these differentiation genes in both (F) OCM1 and (G) C918. (H) ChIP assays showed that ZEB1 bound to MITF-M (melanocyte-specific) and BAP1 promoters, likely to repress their expression. Mean (M) ± standard deviation (SD). ‘*’ Indicates Student’s t-test p ≤ 0.05, ‘**’ Indicates Student’s t-test p ≤ 0.01.