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. 2017 Mar 6;7:72. doi: 10.1038/s41598-017-00067-1

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Inactivation of Rv2170 in the Δicl1 mutant increases generation of CO₂ from stearic acid. Metabolic activity of the bacterial cell was determined using conversion of stearic acid to CO₂. Mtb strains were grown in minimal medium containing [1-¹⁴C] stearic acid and cold 0.05 mM stearic acid: Δicl1 strain, Δicl1:Tn::Rv2170 and complementation strain. CO₂ released from culture was trapped with NaOH and the labeled CO₂ was analyzed with a scintillation counter. The CPM data are shown at different time points. Data are representative from biological triplicates.