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. 2017 Mar 15;7:192. doi: 10.1038/s41598-017-00087-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell number and CFU analysis of BMSCs cultured in static-2D, static-3D and RCCS-3D groups. (a) Proliferation curves of BMSCs in static-2D, static-3D and RCCS-3D groups. The fold increase of cell number was calculated at 0, 1, 3, 5 and 7 day, normalized with the fold increase of cells was set as 0 on the seeding day. The difference on the 7th day was significant by one-way ANOVA analysis (*p < 0.05). (b) Loading efficacy of cells in static-2D, static-3D and RCCS-3D groups. (c) Statistical analysis of colony numbers of BMSCs in each group (n = 3, *p < 0.05). (d–f) Morphology of colonies formed by the cells in static-2D group (d), static-3D group (e) and RCCS-3D group (f) and the colonies were stained with crystal violet.