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. 2017 Jan 31;7:10. doi: 10.1038/s41598-017-00036-8

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© The Author(s) 2017

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Effect of palmitoylation on aggregation of ANCL mutants. HEK293T cells were transfected for 24 hours with wild-type, L115R or ΔL116 EGFP-CSPα constructs, without (A) or with (B) the C(4-7)A substitutions, and with or without HA-zDHHC3 as indicated. Cells were then lysed and examined by immnoblotting with anti-GFP. The position of the molecular weight marker is shown on the left, and the arrows indicate the aggregated (a), palmitoylated monomeric (p) and non-palmitoylated monomeric (np) forms of the protein.