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. 2017 Mar 6;7:78. doi: 10.1038/s41598-017-00094-y

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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IGF2:GFP binding to stem cells. IGF2:GFP was incubated with medaka or zebrafish cell lines and the fluorescence of ligand was analyzed with flow cytometry. (a) Flow cytometric analysis of IGF2:GFP binding to medaka ES cell HX1 and zebrafish ES cell Z428. (b) Binding ability of IGF2:GFP to the stem cells presented by mean fluorescence intensity (MFI).