Figure 6.
qRT-PCR validation of the expression of the predicted biosynthetic genes related to RA in the six organs by RNA-seq. (A) Expression of the genes determined by qRT-PCR. Both DtACT and DtEF1 were selected as the internal controls to normalize the expression. Expression levels of genes in sepals were arbitrarily set to 1 and the other organs were given relative to this. Three biological replicates were performed. A significance threshold of P < 0.05 was considered statistically significant. (B) Correlation of the expression results obtained from qRT-PCR and RNA-seq for biosynthetic genes related to RA.