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. 2017 Mar 3;7:42. doi: 10.1038/s41598-017-00093-z

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression patterns, subcellular localization and transcriptional activity of PsnSHN2. (A) qRT-PCR analysis of the expression levels of PsnSHN2 in different poplar tissues. Actin was used as a control. Error bars represent the standard deviation (SD) of three biological replicates. (B) Subcellular localization of PsnSHN2. Confocal images show the localization of PsnSHN2-GFP in the nuclei of onion epidermal cells. DAPI, a nuclear staining dye; Merged: The merged images of bright-field, GFP and DAPI staining. (C) Transcriptional activation analysis of PsnSHN2 fused with the GAL4 DNA binding domain (GAL4DB) in yeast shows its potential to activate the expression of the His-3 and X-α-Gal reporter genes.