Figure 3.

GSN is the direct target of miR-200a involved in cytoskeleton regulation. (A) The base-pairing interaction of miR-200a seed sequences and GSN, as predicted by bioinformatics analysis. (B) GSN mRNA level measured by quantitative real-time PCR (normalized to GAPDH) and (C) GSN protein level measured by western blotting (normalized to GAPDH) at 48 h after transfection with miR-200a mimic or NC in HepG2/Huh7 cells. (D) Luciferase assay. HEK293T cells were co-transfected with a luciferase construct fused with the wild-type (WT) or mutanted 3′-UTR of GSN and miR-200a mimic or NC. (E) The relative luciferase activity was detected. *P<0.05.