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. 2017 Mar 28;7:477. doi: 10.1038/s41598-017-00490-4

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of drought stress-responsive genes in CaREL1-OX plants. CaREL1-OX plants were exposed to drought stress and the expression levels of ABA-dependent stress-related genes were measured 3 h after detachment using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. The relative expression levels (∆∆CT) of each gene were normalized to the geometric mean of Actin8 as an internal control gene. Data represent the mean ± standard error of three independent experiments. Different letters indicate significant differences between wild-type and transgenic lines (P < 0.05; ANOVA followed by Fisher’s LSD test).