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. 2017 Mar 23;7:337. doi: 10.1038/s41598-017-00407-1

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Tmeff2 gene and Tmeff2-KO mice. (a) Structure of TMEFF2. TMEFF2 has a signal peptide (SP), two follistatin-like domains (FS I, FS II), an EGF-like domain (EGF), a shedding domain (S) and a transmembrane domain (TM). (b) A schematic of Tmeff2-KO mice. A human PLAP cassette was inserted after the start codon in the first exon. (c) The Tmeff2-KO mutant mice are smaller than their wild type littermates. (d) Genotyping results of Tmeff2-KO mice. (e) Tmeff2 mRNA transcription could not be detected by ISH in mutant mice.