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. 2017 Mar 23;7:337. doi: 10.1038/s41598-017-00407-1

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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TMEFF2 is a weak PDGFA-binding protein. (a) Co-IP showed that the affinity between PDGFA and TMEFF2 is lower than that between PDGFA and its natural receptor PDGFRA. (b) TMEFF2 has a weaker effect in stimulating ERK1/2 phosphorylation, compared to PDGFRA, in DMEM media containing 10% FBS or 10 ng/mL PDGFA.