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. 2017 Mar 24;7:404. doi: 10.1038/s41598-017-00402-6

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© The Author(s) 2017

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(A) Calorimetric titration of the preformed CaFADS:ADP:Mg²⁺ complex with FMN. The upper panel shows thermograms for the D298A and WT (inset) titrations and the lower panel displays the corresponding binding isotherms with normalized integrated heats for D298A (●) and WT (○). Experiments carried out in 20 mM PIPES, 10 mM MgCl₂, pH 7.0, at 25 °C.