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. 2017 Mar 24;7:418. doi: 10.1038/s41598-017-00470-8

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MiR-2425-5p promotes bovine MDSCs proliferation. (A) MDSCs were labeled with EdU. EdU-positive cells, green; cell nuclei, blue; magnification, 200×. (B) Percentage of EdU-positive cells, n = 6. (C) Cycle analysis in cells treated with miR-2425-M, miR-2425-M-NC, miR-2425-I, or miR-2425-I-NC for 48 h. (D) Quantification of results shown in (C). Data represent the mean ± SEM (n = 3). (E, F) CCNB1 expression in MDSCs treated with (E) miR-2425-M or (F) miR-2425-I vs. controls at 24 h, 48 h, and 72 h. (G,H) PCNA expression in MDSCs after treatment vs. controls at 24 h, 48 h, and 72 h. *P < 0.05, **P < 0.01, NS: no significant difference.