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. 2017 Mar 24;7:418. doi: 10.1038/s41598-017-00470-8

Figure 3.

Figure 3

MiR-2425-5p inhibits MDSCs differentiation. (A) DES immunofluorescence (green) in MDSCs. The cells were transfected with miR-2425-M, miR-2425-M-NC, miR-2425-I, miR-2425-I-NC, and Normal (MDSCs without any treatment), then differentiated using 2% horse serum for 72 h. Magnification, 200×. (B) Quantification of myotubes according to the DES staining presented in A. (C) MYH3 expression in MDSCs treated with miR-2425-M vs. controls at 24 h, 48 h, and 72 h. (D) MYH3 expression in MDSCs treated with miR-2425-I vs. controls at 24 h, 48 h, and 72 h. *P < 0.05, **P < 0.01, NS: no significant difference.