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. 2017 Mar 24;7:418. doi: 10.1038/s41598-017-00470-8

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MiR-2425-5p regulates RAD9A and MYOG expression. (A) MiR-2425-5p binding to the 3′-UTR of RAD9A and MYOG was examined with luciferase reporter assays performed by psiCHECK-2 vector. (B) RAD9A and MYOG mRNA expression after miR-2425-M transfection at 24 h, 48 h, and 72 h. (C) RAD9A protein expression after transfection of miR-2425-M or miR-2425-I. (D) Quantified data from (C). (E) MYOG protein expression after transfection of miR-2425-M or miR-2425-I. (F) Quantified data from (E). *P < 0.05, **P < 0.01, NS: no significant difference.