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. 2017 Mar 24;7:418. doi: 10.1038/s41598-017-00470-8

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MiR-2425-5p is co-expressed with its host gene, NCKAP5L. (A) Three sgRNAs targeting sites within the NCKAP5L promoter. (B) NCKAP5L expressed in MDSCs after co-transfection of the CRISPRi system. pSPgRNA-N1, pSPgRNA-N2 and pSPgRNA-N3 that represented different inhibitory sequences were co-transfected into MDSCs with dCas9. (C) NCKAP5L expression after co-transfection with dCas9 and pSPgRNA-N2. (D) MiR-2425-5p expression after co-transfection with dCas9 and pSPgRNA-N2. (E) Luciferase activity of pGL3-P1 in MDSCs. *P < 0.05, **P < 0.01, NS: no significant difference.