Figure 3.
The growth disadvantage of ddp-resistant cells is rescued by decreasing ROS levels. (A) Relative ROS levels in HeLa, HeLa/ddp, HGC27, HGC27/ddp, AGS and AGS/ddp cells. (B) Relative ROS levels in HeLa and HeLa/ddp cells under the indicated conditions. (C) Relative clonogenic growth of HeLa and HeLa/ddp cells under the indicated conditions. GSH (4 mM) was added to the medium on the following day. (D) Relative proliferation of HeLa and HeLa/ddp cells under the indicated conditions. Proliferation was determined through CFSE assays after six days. (E) Relative apoptosis of HeLa and HeLa/ddp cells under the indicated conditions. (F) Growth of HeLa and HeLa/ddp cells when cultured together; HeLa cells were labeled with CFSE; HeLa cells and HeLa/ddp cells were plated in 6-well plates at 2 × 105 cells per well in an equal ratio; GSH (4 mM) was added to the medium on the following day. Total cell numbers were counted with a Hand-held Automated Cell Counter, and the ratio of sensitive cells was analyzed via flow cytometry. The error bars represent the s.d. of triplicate wells of a representative experiment.