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. 2017 Mar 27;7:432. doi: 10.1038/s41598-017-00422-2

Figure 4.

Figure 4

Resistant cells show poorer adaptability than sensitive cells in vivo. (A) HeLa and HeLa/ddp cells were implanted and monitored for tumor establishment over a period of 30 days (n = 16 per group). (B) Representative images of tumors. (C) Tumor growth kinetics (n = 16 per group); the error bars indicate s.e.m. (D) Quantification of anti-Ki67 immunohistochemical staining in HeLa and HeLa/ddp tumors (n = 3 mice per group). The error bars indicate s.d. (E) Quantification of TUNEL+ cells per field (n = 3 mice per group). The error bars indicate s.d. (F) Quantification of the average number of CD31+ vessels per field (n = 3 mice per group). The error bars indicate s.d. (G) Representative images of the staining indicated in Fig. E and F. (H) Quantification of the average number of RFP+ cells per field; each dot represents an individual tumor. Tumor cells were isolated when the animal was euthanized, 40 days post-transplantation. The error bars indicate s.d.