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. 2017 Apr 3;7:589. doi: 10.1038/s41598-017-00480-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adult leaf treated with plasma. (a) DCFDA fluorescence image of adult leaf in treated area. Upper panel indicates treatments. Times indicated under H₂DCFDA is exposure time (seconds). Scale bar = 500 um. Representative image were determined from 5 experiments for each conditions. (b) Phenotype of adult leaf after plasma treatment in long day condition for 3 days. Yellow arrow indicates plasma treated area. Heat and Pressure indicates 37 °C heat for 10 seconds and air pressure for 10 seconds, each.