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. 2017 Mar 24;7:409. doi: 10.1038/s41598-017-00466-4

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© The Author(s) 2017

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Smurf1 involves in miR-503 induced osteogenic differentiation in rBMSCs. (A) Expression level of smurf1 was significant down regulated after smurf1 siRNA transfection. Consequently, expression levels of ALP, BMP2 and Runx2 were increased by Smurf1 knockdown. (B) ALP and alizarin red staining were performed 3 days after Smurf1 siRNA transfection under osteogenic induction. Result showed that more ALP positive nodes and calcium nodes were observed in Smurf1 knockdown group. (C,D) The antagomir-503 reversed the upregulation of BMP2 and Runx2 induced by Smurf1 siRNA and it also reduced the promoted effect of Smurf1 knockdown on ALP & Alizarin Red staining. (n = 3, *P < 0.05).