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. 2017 Mar 24;7:409. doi: 10.1038/s41598-017-00466-4

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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X-ray and microCT analysis confirm miR-503 overexpression therapy promote bone formation. (A) X-ray was examined at day 15, 30 and 44. Series of representative radiography were shown. The structure of the distracted bone had already repaired as normal at day 44 postoperation in miR-503 intervention group. A large amount of calluses around the distraction gap still could be found in control group. At 44 days postoperation, the central 150 layers bone tissues within the distraction gap were analyzed by microCT examination. (Vertical interval between red dotted lines represented the distraction gap). (B) Using microCT Quantitative analysis, result showed BV/TV was higher in miR-503 group between the thresholds 158 and 211. (n = 10 each group, *P < 0.05).