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. 2017 Mar 23;7:332. doi: 10.1038/s41598-017-00471-7

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Overexpression of PpSAP1 in transgenic plum. In (a), heterologous expression of PpSAP1 in six independent transgenic lines of plum (35S::PpSAP1 #1, #3, #4, #5, #6, #7) and the wild type (WT). Southern analysis with restriction enzymes HindIII and EcoRI of WT and transgenic lines #1, #5, #6 (b), showing the position of molecular weight markers (kb). In (c), the relative expression of PpSAP1, plum SAP1-like and both genes (PpSAP1 + plum SAP1-like) is shown for three transgenic lines, by using specific primers. An expression value of one is assigned to the WT or the transgenic line #1. Data are means from three biological samples with two technical replicates each, with error bars representing standard deviation. Different letters (a–e) indicate significant difference between samples with a confidence level of 95%.