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. 2017 Mar 24;7:399. doi: 10.1038/s41598-017-00478-0

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fstl1 ^+/− mice have an attenuated pulmonary fibrosis phenotype. (a) Western blot analysis of FSTL1 expression in lung tissues of Fstl1 ^+/− and WT mice 21 days after saline or silica injury. β-tubulin was used as a loading control. (b) H&E staining of lung sections of Fstl1 ^+/− and WT mice 21 days after silica injury. Representative images of the staining are shown. Arrows show inflammatory cells. (n = 6 per group; scale bars, 200 μm). (c) Lung fibrotic score analysis of the lung sections from Fstl1 ^+/− and WT mice 21 days after silica injury (n = 6 per group; *P < 0.05 by one-way ANOVA followed by Student’s t test). The fibrotic area is presented as a percentage. (d) Hydroxyproline contents in lung tissues from Fstl1 ^+/− and WT mice were measured 21 days after saline or silica injury (n = 6 per group; *P < 0.05 by one-way ANOVA followed by Student’s t test). (e) Masson trichrome staining of lung sections of Fstl1 ^+/− and WT mice 21 days after silica treatment. Representative images of the staining are shown (n = 6 per group; scale bars, 200 μm). (f) qRT-PCR analysis of Col1a1 mRNA expression in lung tissues from Fstl1 ^+/− and WT mice 21 days after saline or silica injury (n = 3 per group; ***P < 0.001 by one-way ANOVA followed by Student’s t test). (g) Western blot analysis of type I collagen (Col1) expression in lung tissues from Fstl1 ^+/− and WT mice 21 days after saline or silica injury (n = 3 per group). β-tubulin was used as a loading control. (h) qRT-PCR analysis of fibronectin (Fn1) mRNA expression in lung tissues from Fstl1 ^+/− and WT mice 21 days after saline or silica injury (n = 3 per group; *P < 0.05 by one-way ANOVA followed by Student’s t test).