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. 2017 Mar 27;7:447. doi: 10.1038/s41598-017-00477-1

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© The Author(s) 2017

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Wounding and biomaterial implantation induces acute changes in circulating monocytes. (a) White blood cells were gated on CD11b+SSC^lo expression and categorized as Ly6C^hi, Ly6C^int, and Ly6C^lo monocytes based on Ly6C expression. Ly6G+, dead white blood cells were excluded from analysis. (b) CD11b+SSC^lo monocytes and Ly6C^hi, Ly6C^int, and Ly6C^lo monocytes per milliliter of blood 1 and 3 days post-injury. *p < 0.05 relative to Day 0 blood, n = 5 animals per group. (c) Frequency of CD11b+SSC^lo monocytes and Ly6C^hi, Ly6C^int, and Ly6C^lo monocytes within bone marrow 3 days post-injury. *p < 0.05, n = 4–10 animals per group. Data presented as mean ± S.E.M. Statistical analyses performed using either two-way ANOVA with multiple comparisons or two-tailed t-tests.