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. 2017 Mar 23;7:372. doi: 10.1038/s41598-017-00409-z

Figure 4.

Figure 4

Apigenin acts on GSK3β per se or downstream of GSK3β in the Wnt signaling pathway. (a) Apigenin acts on LRP5 or downstream of LRP5 in the Wnt/β-catenin signaling pathway. P19 cells were transfected with two reporter plasmids together with pLRP5ΔN (grey bar and heavy grey bar), or empty vector (light grey bar), then treated with 50 μM of apigenin, and assayed for dual luciferase activities. The luciferase activity from the expression of empty vector alone was set as 1.0, and the relative activity of other treatment was calculated accordingly. (b) Apigenin acts on DVL or downstream of DVL in the Wnt signal transduction pathway. P19 cells were transfected with two reporter plasmids together with DVL2 plasmid, or empty vector, then treated with different concentrations (0~60 μM) of apigenin, and assayed for dual luciferase activities. The luciferase activity from the expression of DVL alone was set as 100%, and the relative activity of other treatment was calculated accordingly. (c) Apigenin acts on GSK3β or downstream of GSK3β in the Wnt signaling pathway. P19 cells were transfected with two reporter plasmids, incubated in culture medium (CTL) or treated with 20 mM of LiCl as well as different concentrations (0~60 μM) of apigenin in culture medium, and then assayed for dual luciferase activities. The luciferase activity of LiCl treatment alone was set as 100%, and the relative activity of other treatment was calculated accordingly.