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Apigenin decreases the protein levels of cytoplasmic and nuclear β-catenin. HCT-116 cells were treated with different concentrations of apigenin (0, 20, 30, 50 μM) for 22 h. Cells were collected for the isolation of cytosol and nuclear fractions as described in methods. Cytosolic and nuclear lysates were subjected to Western blotting analyses with anti-β-catenin, anti-tubulin, and anti-lamin A. Tubulin served as the cytosolic marker and lamin A served as the nuclear marker.
