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. 2017 Mar 27;7:439. doi: 10.1038/s41598-017-00438-8

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Functional analysis of EgWRI1-1 and EgWRI1-3 in Arabidopsiswri1-4 mutant background. (A) Images of mature seeds of the wild type (ecotype Columbia-0 [Col-0]), mutant (wri1-4), complemented mutant with oil palm Oleosin promoter (EgOle:EgWRI1-1/wri1-4), and non-complemented mutant (EgOle:EgWRI1-3/wri1-4). Size bar: 100 µm. (B–D) Analysis of seed oil traits of Col-0, wri1-4, wri1-4 lines complemented with similar expression level of EgWRI1-1 or EgWRI1-3. Seed oil content (B) and fatty acid (FA) composition (D) were analyzed. (C) The expression level of both AtWRI1 and oil palm WRI1 (EgWRI1/EgWRI3) in complementary lines were measured by qRT-PCR. Error bars correspond to the SD calculated from three technical replicates per pool of 100 seeds.