Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 22;7:311. doi: 10.1038/s41598-017-00434-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

BMDCs raised in hypertonic microenvironment demonstrate clustering of MHCI and SIINFEKL-loaded MHCI molecules. A method alternative to the PLA assay was applied to confirm the receptor-peptide cluster formation upon exposure of BMDCs to high NaCl. (a) Immunofluorescent photomicrographs obtained using double surface staining for MHCI (H-2Db with a secondary Alexa Fluor 488-labeled antibody) and for H2-Kb-SIINFEKL complex (25D1.16 antibody directly fluorescent labeled by APEX Alexa Fluor 555 antibody labeling kit). (b) The statistical data are presented as mean ± SEM (**p ≤ 0.01, n = 16 high power fields).