Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Mar 22;7:311. doi: 10.1038/s41598-017-00434-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

TRIF signaling deficiency abrogates the effect of hypertonicity on CD8⁺ T cell activation. (a) BMDCs of TRIF^lps2/lps2 origin matured in isotonic or indicated NaCl-hypertonic conditions were used in OVA/OTI T cell activation assay as described (a, left). Alternatively, BMDCs matured in isotonic medium were stimulated with NaCl solely during OVA uptake (2 hours) and used in the same assay (a, right). (b–d) Cross-priming of OTI cells by BMDCs raised in different osmolarities, gained from MyD88^−/−, TLR4^−/− and TRIF^−/− mice, respectively. Displayed graphs are representative of minimum two independent experiments (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001; n = 4). (e) BMDCs of TRIF^lps2/lps2 origin matured in 290 mOsm (left column) and 450 mOsm (right column) media were used in PLA-assay as described. Negative control (right column) represents probes with application of only one antibody (25-D1.16.APC antibody was obeyed). The statistical data (f) demonstrate % of PLA-spot or –cluster positive cells within total PLA-positive BMDC population (displayed as mean ± SEM; representative of two pooled experiments, ***p ≤ 0.001, n = 21 high power fields).