Figure 2.

Inhibition of SGK1 in cultured cardiomyocytes by lead compounds. (A) Inhibition of SGK1 activity assessed by GSK3β phosphorylation in CMs infected by Ad.SGK1-CA. (B) Different concentrations of 5377051 were assessed at 48 hours after treatment with immunoblotting (left) and quantitated (right). (C). Effect of SGK1 inhibitors on Akt-induced phosphorylation of GSK3-beta in CMs infected by Ad.-myr-Akt was assessed by immunoblotting. (D) Comparison of potency of 10 micromolar compounds 5377051, 6410136 with the published inhibitor EMD63863. Immunoblots were cut to facilitate the incubation the same samples with multiple antibodies and cropped for clarity. All samples were loaded contiguously as shown. Quantified data are the composite of three independent experiments, each with three technical replicates. Each experimental set was conducted with the same batch of adenovirus.