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. 2017 Mar 22;7:312. doi: 10.1038/s41598-017-00459-3

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of MHT post-treatment on the expression of Claudin-5 and activation of VE-cadherin in the rat lung tissues. (A) Representative immunofluorescence confocal images of rat lung tissue in Control (a), Control + MHT (b), Post-cold 6 h (c), Post-cold 24 h (d), and Post-cold 24 h + MHT (e) group, respectively. Bar = 25 μm. The area within the rectangle in each image in upper panel is enlarged and presented below. Bar = 10 μm. The sections were immunochemically stained for Claudin-5 (green). (B) Western blot for the expression of Claudin-5 in different groups with the quantification of Claudin-5 showing below. (C) Western blot for the VE-cadherin in different groups with the quantification of Claudin-5 showing below. N = 4. Results are presented as mean ± SE. *P < 0.05 vs. Control group; ^#P < 0.05 vs. Post-cold 24 h group.