Figure 2.

Single Point Testing for Compounds Capable of Disrupting. Interaction between EPAC1-CNB and 8-NBD-cAMP in HTS Compounds from the BioAscent library of diverse lead like structures were assessed in 384 well plates at 10 µM with 0.25% DMSO final, n = 1 over two testing days. Responses (% effect) from each compound tested are shown. For the screen a selection cut-off of 15% was calculated using the median plus three times the robust standard deviation (scaled median absolute deviation) of the percentage inhibition across all compound wells, generating 33 hits (indicated with red dots) and a 0.67% hit rate. The screen performed extremely well with consistent levels of maximum (Max) and minimum (Min) control responses, Z’ values and S/B ratios. %CV values were calculated for matching control wells as described (materials and methods) and were below 10%, indicating excellent inter-plate and inter-day variability.