Figure 8.

Binding of FH by B. garinii transformants. Serum adsorption assays were employed to detect binding of FH to viable spirochetes. Wild-type B. garinii (Bg) G1, B. burgdorferi (Bb) LW2, and transformant G1/pBS_CbiA (5 × 10⁸ cells each) were incubated in NHS-EDTA to prevent complement activation, washed, and then bound proteins were eluted using 0.1 M glycine (pH 2.0). Both the last wash (w) and the eluate (e) fractions obtained from each strain were separated by 12.5% SDS-PAGE and transferred to nitrocellulose. A polyclonal anti-FH antibody was used to detect FH by Western blotting. The full-length version is presented in Supplementary Figure S9.