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. 2017 Mar 23;15(5):2940–2948. doi: 10.3892/mmr.2017.6365

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Copyright: © Yin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of PRP formulations on extracellular matrix formation. (A) Western blot analysis was performed to analyze the expression of cartilaginous markers. Reverse transcription-quantitative polymerase chain reaction analysis was performed to detect mRNA expression levels of (B) Col II, (C) aggrecan and (D) SOX9. Data were normalized according to the control group. (E) 1,9-dimethylmethylene blue assay was performed to analyze GAG production. Data are presented as the mean ± standard deviation. *P<0.05, compared with the control group; ^#P<0.05, compared with the L-PRP group. L-PRP, leukocyte and platelet-rich plasma; P-PRP, pure platelet-rich plasma; PDTC, pyrrolidine dithiocarbamate; Col II, collagen II; SOX9, sex-determining region-box Y; GAG, glycosaminoglycan.