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. 2017 Mar 23;7:329. doi: 10.1038/s41598-017-00401-7

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ORF34 physically interacts with ORF24, ORF31, ORF18, ORF23, and ORF66. Cells (293T) were co-transfected with 2xS-tagged ORF34 and 6xMyc-ORF24 (a), 3xFLAG-ORF31 (b), 3xFLAG-ORF18 (c), 3xFLAG-ORF66 (d), 3xFLAG-ORF30 (e), or 6xMyc-ORF23 (f), upper panel) expression plasmids. Cell lysates were subjected to pull-down assays using S-protein-immobilized beads, which captures S-tagged ORF34. The precipitates were immunoblotted with anti-Myc or FLAG antibody to detect their interactions. (e) The pull-down sample was precipitated from cell extracts co-transfected with 2xS-ORF34 and 3xFLAG-ORF31, was applied in the left lane as the positive control. (f), lower panel) The pull-down sample was precipitated from cell extracts co-transfected with 2xS-ORF23 and 6xMyc-ORF34. (a–f) Original images of blotting data are shown in Supplementary Figs S3–S8. (g) Schematic depiction of the interactions between KSHV ORF34 and KSHV-PIC component candidates.