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. 2017 Mar 23;7:329. doi: 10.1038/s41598-017-00401-7

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The interaction of the ORF34 C-terminal region and ORF24 is essential for virus production. The iVero-ΔORF34 cells were transfected with control, ORF34 or a ORF34-deletion mutant plasmid. After 2 days, transfected cells were stimulated with NaB and Dox for 3 days. Progeny KSHV was purified from harvested culture supernatant, and the KSHV genome was quantified by real-time PCR. Viral productivities were assessed using three independent samples, and error bars indicate standard deviations. Statistical significance between each group and the control was analyzed by two-tailed Student’s t-test (N.S.: not significant, P > 0.05).