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. 2017 Mar 28;7:475. doi: 10.1038/s41598-017-00560-7

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PDZ ligand motif of PKCα does not contribute to Ca²⁺ induced PKCα translocation. (A) Exemplified single images of eYFP-PKCα distribution before (Aa1, Ab1, Ac1) and following photolytic Ca²⁺ increase (Aa2, Ab2, Ac2) at the time points marked in the traces to the right. Three different experimental treatments are depicted (Aa-control, Ab-Cyto-D treatment, Ac-nocodazole treatment). Distribution of eYFP-PKCαΔPDZ in HEK293 cell before (Ad1) and after photolytic Ca²⁺ increase (Ad2). Traces were generated from regions of interest in the cytosol (red traces) and on the plasma membrane (blue traces). (B) The statistical summary of plasma membrane accumulation time by fitting an exponential to the upstroke following the flash photolytic Ca²⁺ increase. Numbers given on the bars indicate number of cells in at least 5 independent experiments.