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. 2017 Mar 28;7:475. doi: 10.1038/s41598-017-00560-7

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© The Author(s) 2017

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Cytosolic PKCα mobility is not modulated by the state of the cytoskeleton under resting conditions. (A) Photoconversion of PKCα-Dendra2 by spatially restricted illumination with a focused UV-laser. Inset shows green fluorescence of Dendra2 under resting conditions. The sequence of images depicts the time course of the red PKCα-Dendra2 fluorescence following UV illumination. Time points are given in each image. (B) Fluorescence time course at the point of photoconversion under various experimental conditions (given in the inset). (C) Statistical summary of the fluorescence decay at the point of photoconversion during the experimental conditions given. Numbers given on the bars indicate number of cells in at least 3 independent experiments.