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. 2017 Mar 23;7:352. doi: 10.1038/s41598-017-00535-8

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© The Author(s) 2017

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The results of validation experiments. (a) Chemical structure of NC. (b) Effect of NC on TopI mediated DNA relaxation indifferent concentration. Lane 1, supercoiled pBR322 plasmid DNA; lane 2, DNA + TopI; lane 3–8, DNA + TopI + NC (0.1, 1, 5, 10, 20, 50 μM); lane 9, DNA + TopI + 50 μM camptothecin. (c) Inhibition of TopII relaxation activity. Lane 1, supercoiled pBR322 plasmid DNA; lane 2, DNA + TopII; lane 3–8, DNA + TopII + NC (0.1, 1, 5, 10, 20, 50 μM); lane 9, DNA + TopII + 50 μM etoposide. (d) The effect of adrenaline (5 μg/kg) on arterial blood pressure. (e) NC (0.1 mg/kg) caused reversal of the adrenaline pressor response. (f) The effect of NC (0.01, 0.025, 0.1 mg/kg) on the arterial blood pressure response to adrenaline.