Figure 1.

Generating non-transgenic slmlo1 tomato lines resistant to powdery mildew. (a) The SlMlo1 locus was targeted by two sgRNAs; (b) T0 tomato transformants were tested for the presence of deletions using the PCR band shift assay; (c) Selected T0 transformants genotyped using the PCR band shift assay alongside wild type (WT); (d) SlMlo1 sequencing reads from selected T0 transformants; (e) Leaves of tomato plants inoculated with Oidium neolycopersici (5 weeks post inoculation); (f) PCR genotyping of the T1 generation for the presence T-DNA and the slmlo1 mutation. The agarose gels presented in panels (b and c) were cropped.