Figure 2.

Effects of BPS on meiotic spindle formation during the maturation of porcine oocytes. Representative pictures showing defects in the morphology of spindle organization and chromosome alignment in oocytes after 24 h (A) or 48 h (B) of culture in vitro after BPS (3 nM, 300 nM, and 30 μM) treatment. Green colour indicates α-tubulin, blue indicates DAPI. Scale bar = 10 µm. Percentage of α-tubulin abnormalities after 24 h (n = 83) and 48 h (n = 82) of culture in vitro are presented to the right side of the images. The data are expressed as the mean ± SEM of three independent experiments. Different superscript letters denote statistical significance (P < 0.05).