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. 2017 Mar 28;7:485. doi: 10.1038/s41598-017-00570-5

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© The Author(s) 2017

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Effects of BPS on mRNA expression levels of selected genes and cumulus cell expansion. Effects of BPS (3 nM, 300 nM, and 30 μM) on the relative mRNA expression of (A) ERα, (B) ERβ, and (C) aromatase in oocytes and cumulus cells cultured for 48 h in vitro. The data are expressed from three independent experiments, with a total of n = 150 oocytes per group. Different letters and numbers denote the statistical significance among experimental groups for oocytes and cumulus cells, respectively (P < 0.05). (D) Effects of BPS on HA content in COCs after 24 and 48 h of in vitro culture. Different letters and numbers denote the statistical significance among experimental groups for 24 and 48 h of in vitro culture, respectively (P < 0.05).