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. 2017 Mar 27;7:448. doi: 10.1038/s41598-017-00559-0

Figure 1.

Figure 1

ORS285 ΔnifV mutant displays low in vitro acetylene reducing activity which is restored by homocitrate addition or re-introducing a complete nifV gene. (A) Ethylene production by ORS285 and ORS285 ΔnifV cultures grown in 150 ml vials containing BNM-B medium and 10% acetylene gas at different times post-inoculation. (B) Chromatogram of gas-samples taken from ORS285 (wt) and ORS285 ΔnifV cultures 8 days post-inoculation. WT: dashed line. ΔnifV mutant: solid line. (C) Ethylene production of ORS285 and derivatives grown for 7 days in vacuette® tubes containing BNM-B medium and 10% acetylene gas in the absence or presence of 10 mM homocitrate. The mean amount of produced ethylene per tube (n = 3) is indicated. Error bars represent standard errors of the mean. An ANOVA was performed among the different conditions, followed by Tukey’s post hoc analysis (P < 0.05). The different letters indicate groups that differ significantly.