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. 2017 Mar 27;7:448. doi: 10.1038/s41598-017-00559-0

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© The Author(s) 2017

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ORS285 nifV deletion affects the symbiotic interaction with Aeschynomene nilotica (IRRI 014040). (A) Comparison of the growth of A. nilotica plants inoculated with ORS285 and ORS285 ΔnifV at 28 days post infection (dpi). Non-inoculated plants (ni) were used as control. (B) Mature nodules on A. nilotica plants inoculated with ORS285 and ORS285 ΔnifV. Note the presence of green colored nodules (black arrow) on A. nilotica plants inoculated with the ORS285 ΔnifV strain. (C) Number of root nodules on A. nilotica plants inoculated with Bradyrhizobium ORS285 and Bradyrhizobium ORS285 ΔnifV, respectively. The mean number of nodules per plant (n = 5) at 28 dpi is presented. (D) Acetylene reducing activity of A. nilotica plants inoculated with Bradyrhizobium ORS285 and ORS285 ΔnifV at 28 dpi. The mean amount of produced ethylene per hour and per plant (n = 5) is indicated. In (C) and (D) error bars represent standard errors of the mean and letters represent conditions with significant difference according to the Tukey’s test (P < 0.05).